Indirect positive effects of a sigma factor RpoN deletion on the lactate-based polymer production in Escherichia coli

• Kadoya, Ryosuke

• Kodama, Yu

• Matsumoto, Ken'ichiro

• Taguchi, Seiichi

抄録

The production of bacterial polyesters, polyhydroxyalkanoates (PHAs), has been improved by several rational approaches such as overexpression and/or engineering of the enzymes directly related to PHA biosynthetic pathways. In this study, a new approach at transcription level has been applied to a new category of the copolymer of lactate (LA) and 3-hydroxybutyrate (3HB), P(LA-co-3HB). When the 4 disrupting mutants of sigma factors in Escherichia coli, rpoN, rpoS, fliA, fecI, were used as platforms for production of P(LA-co-3HB), increases in the production level and LA fraction of the copolymer were observed for the mutant strain with rpoN disruption. These positive impacts on the polymer production were caused in an indirect manner via changes in the multiple genes governed by RpoN. A genome-wide engineering by sigma factors would be a versatile approach for the production of value-added products of interest and available for combination with the other beneficial tools.

収録刊行物

• Bioengineered

  Bioengineered 6 (5), 307-311, 2015

  Taylor & Francis

キーワード

• biobased plastic
• Escherichia coli
• polyhydroxyalkanoate
• polyhydroxybutyrate
• poly(lactic acid)
• sigma factor

詳細情報

• CRID: 1050564288972720128
• NII論文ID: 120005670608
• HANDLE: 2115/60235
• ISSN: 21655979
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles