The cell competition-based high-throughput screening identifies small compounds that promote the elimination of RasV12-transformed cells from epithelia
抄録
Recent studies have revealed that cell competition can occur between normal and transformed epithelial cells; normal epithelial cells recognize the presence of the neighboring transformed cells and actively eliminate them from epithelial tissues. Here, we have established a brand-new high-throughput screening platform that targets cell competition. By using this platform, we have identified Rebeccamycin as a hit compound that specifically promotes elimination of RasV12-transformed cells from the epithelium, though after longer treatment it shows substantial cytotoxic effect against normal epithelial cells. Among several Rebeccamycin-derivative compounds, we have found that VC1-8 has least cytotoxicity against normal cells but shows the comparable effect on the elimination of transformed cells. This cell competition-promoting activity of VC1-8 is observed both in vitro and ex vivo. These data demonstrate that the cell competition-based screening is a promising tool for the establishment of a novel type of cancer preventive medicine.
収録刊行物
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- Scientific reports
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Scientific reports 5 15336-, 2015-10-21
Nature Publishing Group
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詳細情報 詳細情報について
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- CRID
- 1050001202927744768
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- NII論文ID
- 120005674073
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- HANDLE
- 2115/60284
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- ISSN
- 20452322
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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