分泌型免疫グロブリンＡが認識する 腸内細菌種の同定

• 鶴田, 剛司

書誌事項

タイトル別名

• Identification of commensal bacteria coated with secretory immunoglobulin A

抄録

A part of commensal intestinal bacteria in mammal including human, mouse, bovine and pig are coated with secretory immunoglobulin A (S‒IgA). It has been suggested from our previous research that S‒IgA coating of commensal bacteria occur in bacterial group specific manner in human and mouse intestine. Thus, identification of S‒IgA‒coated bacterial genera/species would certainly help to elucidate the interaction between S‒IgA and commensal intestinal bacteria. However, the method to identify the genera/species of S‒IgA‒coated bacteria has not been established. To identify S‒IgA‒coated bacterial composition, we developed the method combining immunohistochemical detection of S‒IgA and subsequent 16S rRNA targeted fluorescence in situ hybridization (FISH) analysis. Furthermore, human and mice fecal S‒IgA coated bacterial composition was evaluated by this newly developed method with ten frequently‒used FISH probes. Fecal S‒IgA‒coated bacterial composition was successfully analyzed with this method and this analysis suggested that Enterobacteriaceae was preferably coated with S‒IgA whereas Bacteroides/Prevotella and Lactobacillus/ Enterococcus groups seemed to be poorly coated with S‒IgA. This method will be applied to confirm whether interaction between S‒IgA and commensal intestinal bacteria relate to symptom of inflammatory bowel diseases.

収録刊行物

• 岡山大学農学部学術報告

  岡山大学農学部学術報告 105 35-39, 2016-02-01

  岡山大学農学部

キーワード

• Secretory immunoglobulin A
• commensal intestinal bacteria
• fluorescence in situ hybridization

詳細情報

• CRID: 1050846637266226688
• NII論文ID: 120005697286
• ISSN: 21867755
• Web Site: https://ousar.lib.okayama-u.ac.jp/53971
• 本文言語コード: ja
• 資料種別: departmental bulletin paper
• データソース種別:
  • IRDB
  • CiNii Articles