Improvement of Porcine SCNT Embryo Development Using Histone Deacetylase Inhibitors

• Lee Jin-Hee

  Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University

• Chun Ju Lan

  Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University

• Lee Ji Hye

  Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University

• Kim Keun Jung

  Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University

• Kim Eun Young

  Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University

• Han Kil-Woo

  Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University

• 後藤 貴文

  九州大学大学院農学研究院農学部附属農場高原農業実験実習場

• Kim Min Kyu

  Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University

抄録

Somatic cell nuclear transfer (SCNT) has been used in crucial and potential fields of the animal reproduction. Despite the successful birth of cloned animals, the cloning efficiency of SCNT has been still low. Abnormal epigenetic reprogramming has been reported as the cause of the low efficiency of SCNT in cloned embryos. Here, the study aimed to improve the developmental competence of porcine SCNT embryos using Trichostatin A (TSA) and Scriptaid which are well known as histone deacetylase inhibitors that would enhance epigenetic reprograming in cloned embryos by suppressing the event of histone deacetylation. Moreover, this study tested whether Scriptaid would be a substitute for TSA because it has been suggested that TSA is involved in malformation of cloned embryos. Various concentrations of Scriptaid were tested and 500 nM Scriptaid treatment resulted in a significant improvement of the cloned embryo during development regarding the blastocyst formation rates. When cloned embryos were treated with 50 nM TSA or/and 500 nM Scriptaid for 15 h or 24 h, the blastocyst rates of reconstructed embryos were increased in comparison to the untreated control group. However, there was no dose–dependent difference among groups. When donor cells were treated with 50 nM TSA or/and 500 nM Scriptaid for 4 h the blastocyst rates of reconstructed embryos were increased in comparison with the untreated control group. Moreover the expression levels of histone deacetylase 1 (HDAC1) and histone deacetylase 2 (HDAC2) were decreased with TSA and/or Scriptaid treatment. In conclusion, TSA and/or Scriptaid treatments significantly increased the developmental competence of porcine SCNT embryos. In addition, Scriptaid improved the development of SCNT embryos regardless of the TSA treatment. Therefore, Scriptaid would be an alternative additive to improve the development competence of cloned embryos after SCNT.

収録刊行物

• Journal of the Faculty of Agriculture, Kyushu University

  Journal of the Faculty of Agriculture, Kyushu University 61 (1), 115-120, 2016-02-29

  九州大学大学院農学研究院

キーワード

• histone deacetylase inhibitor
• porcine embryo
• Scriptaid
• SCNT
• TSA

詳細情報

• CRID: 1390009224684786688
• NII論文ID: 120005725050
• NII書誌ID: AA00247166
• DOI: 10.5109/1564091
• HANDLE: 2324/1564091
• ISSN: 00236152
• 本文言語コード: en
• データソース種別:
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  • IRDB
  • Crossref
  • CiNii Articles