Clock gene expressions are altered by co-culture of gingival fibroblasts and oral cancer cells
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- Furudate Ken
- Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine
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- Matsumiya Tomoh
- Department of Vascular Biology, Institute of Brain Science, Hirosaki University Graduate School of Medicine
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- Hayakari Ryo
- Department of Vascular Biology, Institute of Brain Science, Hirosaki University Graduate School of Medicine
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- Xing Fei
- Department of Vascular Biology, Institute of Brain Science, Hirosaki University Graduate School of Medicine
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- Kubota Kosei
- Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine
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- Sakaki Hirotaka
- Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine
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- Tamura Yoshihiro
- Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine
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- Kijima Hiroshi
- Department of Pathology and Bioscience, Hirosaki University Graduate School of Medicine
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- Imaizumi Tadaatsu
- Department of Vascular Biology, Institute of Brain Science, Hirosaki University Graduate School of Medicine
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- Kimura Hiroto
- Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine
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- Kobayashi Wataru
- Department of Dentistry and Oral Surgery, Hirosaki University Graduate School of Medicine
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抄録
Tumor microenvironment is related to growth, survival, invasion, and metastasis of tumor cells. Several studies have proved that stromal fibroblasts play an important role in the tumor microenvironment to convert cancer-associated fibroblast (CAFs). Clock genes are known to regulate circadian rhythms, angiogenesis, and immunoreaction. In addition, clock genes play an important role in cancer development. However, little has been shown about how these clock genes function in the tumor microenvironment. In the present study, we investigated to evaluate the effect of co-culture fibroblasts with oral cancer cells on the expression of clock genes. Following the co-culture of human primary fibroblasts with human gingival carcinoma Ca9-22 cells, the expression levels of clock genes were analyzed by real-time quantitative PCR. We found that the rhythmic expression of clock genes were altered, enhanced, or disappeared by the co-culture. Such effect was observed not only in fibroblasts in the presence of Ca9-22 cells but also in Ca9-22 cells in the presence of fibroblasts. Our results suggested that clock genes might affect an important role in the tumor microenvironment.
収録刊行物
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- 弘前医学
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弘前医学 66 (2-4), 135-142, 2016
弘前大学大学院医学研究科・弘前医学会
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詳細情報 詳細情報について
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- CRID
- 1390006740596391296
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- NII論文ID
- 130008046380
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- NII書誌ID
- AN00211444
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- ISSN
- 24344656
- 04391721
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- HANDLE
- 10129/5809
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- NDL書誌ID
- 027278177
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可