<Originals> Synergistic effect of the combination of corticosteroids and long acting-β_2 agonists on inhibiting the in vitro proliferation of T cells isolated from asthmatic patients

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[Abstract] The clinical efficacy of inhaled corticosteroids (ICS) combined with long-acting , β_2- agonists (LABA) has been widely demonstrated in asthma. In order to validate the benefits of the combination of these agents in vitro, we studied the inhibitory effect of fluticasone propionate (FP) or salmeterol either alone or incombination on the proliferation of T cells. In the present study, peripheral blood mononuclear cells (PBMCs) from asthmatic patients were stimulated with phytohemagglutinin (PHA) for four days in the presence of FP and/or salmeterol. We assessed cell proliferation by quantifying the fluorescence of carboxyfluorescein diacetate succinimidyl ester (CFSE) in CD3^+ cells using flow cytometry. A high concentration (10^<-8>M), but not a low concentration (10^<-12>M) of FP alone inhibited PHA-induced T cell proliferation, while salmeterol alone did not have any effect. The combination of FP (10^<-12>M) and salmeterol inhibited PHA-induced T cell proliferation, however, the combination of FP (10^<-12>M) and salbutamol, which is another shortacting β_2-agonist, did not inhibit PHA-induced T cell proliferation. This study shows that the combination of a low concentration of FP and salmeterol synergistically inhibits lymphocyte proliferation in vitro. This novel synergistic interaction of ICS and LABA may partly explain the benefits that result when these agents are given in combination to treat asthma.

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