SsODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

• Yoshimi, Kazuto

• Kunihiro, Yayoi

• Kaneko, Takehito

• Nagahora, Hitoshi

• Voigt, Birger

• Mashimo, Tomoji

抄録

The CRISPR-Cas system is a powerful tool for generating genetically modified animals; however, targeted knock-in (KI) via homologous recombination remains difficult in zygotes. Here we show efficient gene KI in rats by combining CRISPR-Cas with single-stranded oligodeoxynucleotides (ssODNs). First, a 1-kb ssODN co-injected with guide RNA (gRNA) and Cas9 messenger RNA produce GFP-KI at the rat Thy1 locus. Then, two gRNAs with two 80-bp ssODNs direct efficient integration of a 5.5-kb CAG-GFP vector into the Rosa26 locus via ssODN-mediated end joining. This protocol also achieves KI of a 200-kb BAC containing the human SIRPA locus, concomitantly knocking out the rat Sirpa gene. Finally, three gRNAs and two ssODNs replace 58-kb of the rat Cyp2d cluster with a 6.2-kb human CYP2D6 gene. These ssODN-mediated KI protocols can be applied to any target site with any donor vector without the need to construct homology arms, thus simplifying genome engineering in living organisms.

収録刊行物

• Nature Communications

  Nature Communications 7 2016-01-20

  Nature Publishing Group

キーワード

• Biological sciences
• Molecular biology
• Genetics

詳細情報

• CRID: 1050845760760120704
• NII論文ID: 120005743652
• ISSN: 20411723
• HANDLE: 2433/210211
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles