Leader-Containing Uncapped Viral Transcript Activates RIG-I in Antiviral Stress Granules

• 石館, 文善

• 加藤, 博己

• 藤田, 尚志

• Onoguchi, Kazuhide

• Ishidate, Fumiyoshi

• Fujiwara, Takahiro

• Yoneyama, Mitsutoshi

• Kato, Hiroki

• Fujita, Takashi

抄録

RIG-I triggers antiviral responses by recognizing viral RNA (vRNA) in the cytoplasm. However, the spatio-temporal dynamics of vRNA sensing and signal transduction remain elusive. We investigated the time course of events in cells infected with Newcastle disease virus (NDV), a non-segmented negative-strand RNA virus. RIG-I was recruited to viral replication complexes (vRC) and triggered minimal primary type I interferon (IFN) production. RIG-I subsequently localized to antiviral stress granules (avSG) induced after vRC formation. The inhibition of avSG attenuated secondary IFN production, suggesting avSG as a platform for efficient vRNA detection. avSG selectively captured positive-strand vRNA, and poly(A)+ RNA induced IFN production. Further investigations suggested that uncapped vRNA derived from read-through transcription was sensed by RIG-I in avSG. These results highlight how viral infections stimulate host stress responses, thereby selectively recruiting uncapped vRNA to avSG, in which RIG-I and other components cooperate in an efficient antiviral program.

[Correction]5 Apr 2016: Oh SW, Onomoto K, Wakimoto M, Onoguchi K, Ishidate F, et al. (2016) Correction: Leader-Containing Uncapped Viral Transcript Activates RIG-I in Antiviral Stress Granules. PLOS Pathogens 12(4): e1005563. https://doi.org/10.1371/journal.ppat.1005563

収録刊行物

• PLOS Pathogens

  PLOS Pathogens 12 (2), 2016-02-10

  Public Library of Science

詳細情報

• CRID: 1050282677826060160
• NII論文ID: 120005763384
• ISSN: 15537374
• HANDLE: 2433/214491
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles