Long-term expression of miRNA for RNA interference using a novel vector system based on a negative-strand RNA virus

• 本田, 知之

• 牧野, 晶子

• 朝長, 啓造

• Matsumoto, Yusuke

• Makino, Akiko

• Tomonaga, Keizo

抄録

RNA interference (RNAi) has emerged as a promising technique for gene therapy. However, the safe and long-term expression of small RNA molecules is a major concern for the application of RNAi therapies in vivo. Borna disease virus (BDV), a non-segmented, negative-strand RNA virus, establishes a persistent infection without obvious cytopathic effects. Unique among animal non-retroviral RNA viruses, BDV persistently establishes a long-lasting persistent infection in the nucleus. These features make BDV ideal for RNA virus vector persistently expressing small RNAs. Here, we demonstrated that the recombinant BDV (rBDV) containing the miR-155 precursor, rBDV-miR-155, persistently expressed miR-155 and efficiently silenced its target gene. The stem region of the miR-155 precursor in rBDV-miR-155 was replaceable by any miRNA sequences of interest and that such rBDVs efficiently silence the expression of target genes. Collectively, BDV vector would be a novel RNA virus vector enabling the long-term expression of miRNAs for RNAi therapies.

収録刊行物

• Scientific Reports

  Scientific Reports 6 2016-05-18

  Nature Publishing Group

詳細情報

• CRID: 1050845760765729152
• NII論文ID: 120005770739
• ISSN: 20452322
• HANDLE: 2433/215183
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles