Truncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA viruses

DOI 機関リポジトリ HANDLE HANDLE HANDLE ほか2件をすべて表示 一部だけ表示 被引用文献3件 参考文献64件 オープンアクセス

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RNA viruses use various strategies to condense their genetic information into small genomes. Potyviruses not only use the polyprotein strategy, but also embed an open reading frame, pipo, in the P3 cistron in the-1 reading frame. PIPO is expressed as a fusion protein with the N-terminal half of P3 (P3N-PIPO) via transcriptional slippage of viral RNA-dependent RNA polymerase (RdRp). We herein show that clover yellow vein virus (ClYVV) produces a previously unidentified factor, P3N-ALT, in the +1 reading frame via transcriptional slippage at a conserved G1-2A6-7 motif, as is the case for P3N-PIPO. The translation of P3N-ALT terminates soon, and it is considered to be a C-terminal truncated form of P3. In planta experiments indicate that P3N-ALT functions in cell-to-cell movement along with P3N-PIPO. Hence, all three reading frames are used to produce functional proteins. Deep sequencing of ClYVV RNA from infected plants endorses the slippage by viral RdRp. Our findings unveil a virus strategy that optimizes the coding capacity.

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  • Scientific Reports

    Scientific Reports 6 21411-1-21411-14, 2016-02-22

    Nature Publishing Group

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