The cardiorespiratory responses to inhalation and pentobarbital anesthesia in the mouse

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  • Han Chong
    Department of Pharmacology, Hirosaki University Graduates School of Medicine
  • Ogata Yoshiki
    Department of Pharmacology, Hirosaki University Graduates School of Medicine
  • Niwa Hidetoshi
    Department of Anesthesiology, Hirosaki University Graduates School of Medicine
  • Kushikata Tetsuya
    Department of Anesthesiology, Hirosaki University Graduates School of Medicine
  • Watanabe Hiroyuki
    Department of Internal Medicine Division of Cardiovascular and Respiratory Medicine, Akita University School of Medicine
  • Imaizumi Tadaatsu
    Department of Vascular Biology, Institute of Brain Science, Hirosaki University Graduates School of Medicine
  • Hirota Kazuyoshi
    Department of Anesthesiology, Hirosaki University Graduates School of Medicine
  • Ono Kyouichi
    Department of Cellphysiology, Akita University School of Medicine
  • Ohba Takayoshi
    Department of Cellphysiology, Akita University School of Medicine
  • Murakami Manabu
    Department of Pharmacology, Hirosaki University Graduates School of Medicine

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抄録

    Transgenic mice experiments have become increasingly popular to research human inherited disease. However, a number of Japanese researchers have difficulty with the selection of anesthesia, after the classification of ketamine, probably the most used anesthesia, as a narcotic drug in 2006. Therefore, we compared the effects of inhalation anesthesia (2% of isoflurane, sevoflurane and enflurane) and intraperitoneal pentobarbital anesthesia (50mg/kg) on the electrocardiogram (ECG) and blood oxygen saturation (SPO₂) of mice. With inhalation anesthesia, the heart rate (HR) and SPO₂ were within an acceptable range. In contrast, the HR significantly decreased after initiation of pentobarbital anesthesia, and gradually returned to a low rate. Importantly, pentobarbital anesthesia significantly lowered SPO₂, and heart rate variability analysis showed unstable beat-to-beat intervals during pentobarbital anesthesia, suggesting that inhalation anesthesia is more suitable for evaluation of cardiorespiratory responses than pentobarbital anesthesia. During anesthesia, propranolol, a β-adrenergic blocker, significantly decreased heart rate. Atropine, a parasympathetic blocker, also significantly increased heart rate. Our data suggest that inhalation anesthesia is suitable for cardiorespiratory analysis in mice.

収録刊行物

  • 弘前医学

    弘前医学 67 (1), 77-85, 2016

    弘前大学大学院医学研究科・弘前医学会

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