An RNA-dependent RNA polymerase gene in bat genomes derived from an ancient negative-strand RNA virus.

• 朝長, 啓造

• Kobayashi, Yuki

• Honda, Tomoyuki

• Fujino, Kan

• Akasaka, Takumi

• Kohl, Claudia

• Wibbelt, Gudrun

• Mühldorfer, Kristin

• Kurth, Andreas

• Müller, Marcel A.

• Corman, Victor M.

• Gillich, Nadine

• Suzuki, Yoshiyuki

• Schwemmle, Martin

• Tomonaga, Keizo

抄録

Endogenous bornavirus-like L (EBLL) elements are inheritable sequences derived from ancient bornavirus L genes that encode a viral RNA-dependent RNA polymerase (RdRp) in many eukaryotic genomes. Here, we demonstrate that bats of the genus Eptesicus have preserved for more than 11. 8 million years an EBLL element named eEBLL-1, which has an intact open reading frame of 1, 718 codons. The eEBLL-1 coding sequence revealed that functional motifs essential for mononegaviral RdRp activity are well conserved in the EBLL-1 genes. Genetic analyses showed that natural selection operated on eEBLL-1 during the evolution of Eptesicus. Notably, we detected efficient transcription of eEBLL-1 in tissues from Eptesicus bats. To the best of our knowledge, this study is the first report showing that the eukaryotic genome has gained a riboviral polymerase gene from an ancient virus that has the potential to encode a functional RdRp.

収録刊行物

• Scientific reports

  Scientific reports 6 2016-05-13

  Springer Nature

詳細情報

• CRID: 1050564285789146880
• NII論文ID: 120005822794
• ISSN: 20452322
• HANDLE: 2433/216228
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles
  • KAKEN