In Vivo Mouse Intervertebral Disc Degeneration Model Based on a New Histological Classification

• Ohnishi, Takashi

• Sudo, Hideki

• Iwasaki, Koji

• Tsujimoto, Takeru

• Ito, Yoichi M.

• Iwasaki, Norimasa

抄録

Although human intervertebral disc degeneration can lead to several spinal diseases, its pathogenesis remains unclear. This study aimed to create a new histological classification applicable to an in vivo mouse intervertebral disc degeneration model induced by needle puncture. One hundred six mice were operated and the L4/5 intervertebral disc was punctured with a 35- or 33-gauge needle. Micro-computed tomography scanning was performed, and the punctured region was confirmed. Evaluation was performed by using magnetic resonance imaging and histology by employing our classification scoring system. Our histological classification scores correlated well with the findings of magnetic resonance imaging and could detect degenerative progression, irrespective of the punctured region. However, the magnetic resonance imaging analysis revealed that there was no significant degenerative intervertebral disc change between the ventrally punctured and non-punctured control groups. To induce significant degeneration in the lumbar intervertebral discs, the central or dorsal region should be punctured instead of the ventral region.

収録刊行物

• PLoS ONE

  PLoS ONE 11 (8), e0160486-, 2016-08-02

  The Public Library of Science

詳細情報

• CRID: 1050564288974113920
• NII論文ID: 120005851049
• HANDLE: 2115/63179
• ISSN: 19326203
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles