〈論文・報告〉miRNA様siRNAによる遺伝子サイレンシング効果

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タイトル別名
  • Gene Silencing by miRNA-like siRNA
  • miRNA様siRNAによる遺伝子サイレンシング効果
  • miRNA ヨウ siRNA ニ ヨル イデンシ サイレンシング コウカ

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RNA interference (RNAi) is a genetic regulation system induced by small RNAs. There are two pathways in RNAi, one is induced by small interfering RNA (siRNA) and the other by micro RNA (miRNA). siRNA is a full matched 21-23nt dsRNA and thought to evolutionally develop as a biological defense mechanism against RNA viruses. On the other hand, miRNA is an endogenously transcribed 20-25nt dsRNA having two or three mismatches and is involved in various biological processes. Based on structural and mechanistic similarities and differences between siRNA and miRNA, RNAi efficiencies of siRNAs with 3 mismatches at 2, 10 and 14 positions and siRNAs with 2 mismatches at 2 and 14 positions were evaluated. As a result, siRNA bearing a mismatch at central position showed weaker RNAi effect than siRNAs with no mismatch at central position. It can be concluded that RNAi by Ago2-RISC is stronger than that by Ago1-RISC.

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