SOX10-Nano-Lantern Reporter Human iPS Cells; A Versatile Tool for Neural Crest Research

• 池谷, 真

• Ohi, Hiromi

• Shibata, Mitsuaki

• Ikeya, Makoto

• Ueno, Morio

• Sotozono, Chie

• Kinoshita, Shigeru

• Sato, Takahiko

抄録

The neural crest is a source to produce multipotent neural crest stem cells that have a potential to differentiate into diverse cell types. The transcription factor SOX10 is expressed through early neural crest progenitors and stem cells in vertebrates. Here we report the generation of SOX10-Nano-lantern (NL) reporter human induced pluripotent stem cells (hiPS) by using CRISPR/Cas9 systems, that are beneficial to investigate the generation and maintenance of neural crest progenitor cells. SOX10-NL positive cells are produced transiently from hiPS cells by treatment with TGFβ inhibitor SB431542 and GSK3 inhibitor CHIR99021. We found that all SOX10-NL-positive cells expressed an early neural crest marker NGFR, however SOX10-NL-positive cells purified from differentiated hiPS cells progressively attenuate their NL-expression under proliferation. We therefore attempted to maintain SOX10-NL-positive cells with additional signaling on the plane and sphere culture conditions. These SOX10-NL cells provide us to investigate mass culture with neural crest cells for stem cell research.

収録刊行物

• PLOS ONE

  PLOS ONE 12 (1), 2017-01-20

  Public Library of Science

詳細情報

• CRID: 1050845760771227904
• NII論文ID: 120005980867
• ISSN: 19326203
• HANDLE: 2433/218475
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles