Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane

• Fujiwara, T. K.

  Center for Meso-Bio Single-Molecule Imaging, Institute for Integrated Cell-Material Sciences

• Iwasawa, K.

  Institute for Frontier Medical Sciences, Kyoto University

• Kalay, Z.

  Institute for Integrated Cell-Material Sciences

• Tsunoyama, T. A.

• Watanabe, Y.

• Umemura, Y. M.

• Murakoshi, H.

• Suzuki, K. G. N.

• Nemoto, Y. L.

• Morone, N.

• Kusumi, A.

• Patricia Bassereau

  editor

抄録

The mechanisms by which the diffusion rate in the plasma membrane (PM) is regulated remain unresolved, despite their importance in spatially regulating the reaction rates in the PM. Proposed models include entrapment in nanoscale noncontiguous domains found in PtK2 cells, slow diffusion due to crowding, and actin-induced compartmentalization. Here, by applying single-particle tracking at high time resolutions, mainly to the PtK2-cell PM, we found confined diffusion plus hop movements (termed "hop diffusion") for both a nonraft phospholipid and a transmembrane protein, transferrin receptor, and equal compartment sizes for these two molecules in all five of the cell lines used here (actual sizes were cell dependent), even after treatment with actin-modulating drugs. The cross-section size and the cytoplasmic domain size both affected the hop frequency. Electron tomography identified the actin-based membrane skeleton (MSK) located within 8.8 nm from the PM cytoplasmic surface of PtK2 cells and demonstrated that the MSK mesh size was the same as the compartment size for PM molecular diffusion. The extracellular matrix and extracellular domains of membrane proteins were not involved in hop diffusion. These results support a model of anchored TM-protein pickets lining actin-based MSK as a major mechanism for regulating diffusion.

収録刊行物

• Molecular Biology of the Cell

  Molecular Biology of the Cell 27 (7), 1101-1119, 2016-04-01

  American Society for Cell Biology (ASCB)

キーワード

• Cell Biology
• Molecular Biology

詳細情報

• CRID: 1050001335841232512
• NII論文ID: 120005997940
• ISSN: 10591524
• ISSN: 19394586
• DOI: 10.1091/mbc.e15-04-0186
• HANDLE: 2433/218974
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN