Vinculin association with actin cytoskeleton is necessary for stiffness-dependent regulation of vinculin behavior

• 市川, 尚文

• 木村, 泰久

• 植田, 和光

• 木岡, 紀幸

• Kioka, Noriyuki

• Nic D. Leipzig

  editor

抄録

The extracellular matrix (ECM) is a major regulator of cell behavior. Recent studies have indicated the importance of the physical properties of the ECM, including its stiffness, for cell migration and differentiation. Using actomyosin-generated forces, cells pull the ECM and sense stiffness via cell-ECM adhesion structures called focal adhesions (FAs). Vinculin, an actin-binding FA protein, has emerged as a major player in FA-mediated mechanotransduction. Although vinculin is important for sensing ECM stiffness, the role of vinculin binding to actin in the ECM stiffness-mediated regulation of vinculin behavior remains unknown. Here, we show that an actin binding-deficient mutation disrupts the ECM stiffness-dependent regulation of CSB (cytoskeleton stabilization buffer) resistance and the stable localization of vinculin. These results suggest that the vinculin-actin interaction participates in FA-mediated mechanotransduction.

収録刊行物

• PLOS ONE

  PLOS ONE 12 (4), e0175324-, 2017-04-01

  Public Library of Science

キーワード

• Multidisciplinary

詳細情報

• CRID: 1050845760779297792
• NII論文ID: 120006309292
• ISSN: 19326203
• DOI: 10.1371/journal.pone.0175324
• HANDLE: 2433/225270
• Web Site: http://dx.plos.org/10.1371/journal.pone.0175324
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • Crossref
  • CiNii Articles
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