〔報告〕高松塚古墳・キトラ古墳石室内の微生物分離株のアルコール系殺菌剤資化性試験

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タイトル別名
  • 高松塚古墳・キトラ古墳石室内の微生物分離株のアルコール系殺菌剤資化性試験
  • タカマツズカ コフン キトラ コフン セキシツナイ ノ ビセイブツ ブンリカブ ノ アルコールケイ サッキンザイシカセイ シケン

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In Takamatsuzuka and Kitora Tumuli, para-formaldehyde fumigation had been used to kill microorganisms. But ethanol or isopropanol was also occasionally used for the removal of colonies of microorganisms which contaminated the murals as dense colonies survived even after para-formaldehyde fumigation. At high concentration, these alcohols are strong disinfectants that do not affect pigments of the mural paintings. Also, para-formaldehyde is presently known as a potent carcinogen, so lower toxic disinfectants were desired for use. However, there was a concern that microorganisms might use such alcohols as carbon sources when the alcohols are diluted to a very low concentration. In order to test this possibility, we examined the growth of strains of microorganisms isolated from Takamatsuzuka and Kitora Tumuli. Strains were cultured in minimum liquid media containing low concentration of each alcohol (approximately 0.5% or 1% in volume) as a single carbon source. All 15 fungal strains tested could utilize ethanol (approximately 0.5% and 1% in volume) as a single carbon source. On the other hand, fungal strains did not utilize isopropanol so effectively as ethanol except for strains of Acremonium (sect Gliomastix) sp. All 5 yeast strains tested could utilize ethanol (approximately 0.5% and 1% in volume). But they did not utilize isopropanol. Nine bacterial strains out of 10 could utilize both ethanol and isopropanol (approximately 0.5% and 1% in volume). Only one strain of Microbacterium sp. did not utilize either of them. Ethanol and isopropanol were supposed to be mild disinfectants to pigments of murals, but when they are diluted to a very low concentration, they might act as carbon sources, ie. nutrients for microorganisms.

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