Effect of Reverse Vessel Remodeling on Regression of Coronary Atherosclerosis in Patients Treated With Aggressive Lipid- and Blood Pressure-Lowering Therapy　- Insight From MILLION Study.

DOI DOI DOI 機関リポジトリ Web Site ほか3件をすべて表示 一部だけ表示 被引用文献5件 参考文献44件
  • Yamagishi Masakazu
    Divisions of Cardiovascular Medicine and Bioscience, National Cardiovascular Center and Research Institute
  • Higashikata Takeo
    Divisions of Biotechnology in Bioscience, National Cardiovascular Center and Research Institute
  • Ishibashi-Ueda Hatsue
    Divisions of Pathology, National Cardiovascular Center and Research Institute
  • Sasaki Hiroaki
    Divisions of Cardiovascular Surgery, National Cardiovascular Center and Research Institute
  • Ogino Hitoshi
    Divisions of Cardiovascular Surgery, National Cardiovascular Center and Research Institute
  • Iihara Koji
    Divisions of Neurosurgery, National Cardiovascular Center and Research Institute
  • Miyamoto Susumu
    Divisions of Neurosurgery, National Cardiovascular Center and Research Institute
  • Nagaya Noritoshi
    Divisions of Regenerative Medicine and Tissue Engineering, National Cardiovascular Center and Research Institute
  • Tomoike Hitonobu
    Divisions of Cardiovascular Medicine and Bioscience, National Cardiovascular Center and Research Institute
  • Sakamoto Aiji
    Divisions of Biotechnology in Bioscience, National Cardiovascular Center and Research Institute

書誌事項

タイトル別名
  • Sustained Upregulation of Inflammatory Chemokine and Its Receptor in Aneurysmal and Occlusive Atherosclerotic Disease Results From Tissue Analysis With cDNA Macroarray and Real-Time Reverse Transcriptional Polymerase Chain Reaction Methods
  • Results From Tissue Analysis With cDNA Macroarray and Real-Time Reverse Transcriptional Polymerase Chain Reaction Methods

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Background Although cytokines are known to be pivotal in the development of atherosclerotic diseases, few data exist regarding their expressions in the established stages such as aneurysmal or occlusive lesions. Therefore, in the present study the gene expression levels of cytokine-related substances in abdominal aortic aneurysm (AAA) and carotid artery stenosis (CAS) were determined using cDNA macroarray and real-time reverse transcriptase polymerase chain reaction (RT-PCR) methods. Methods and Results Tissue samples were obtained from 31 patients with AAA and 24 with CAS. The array-specific [33P]-labeled cDNA probe mixture synthesized from 2.5 μg total RNA with gene-specific primers was hybridized with nylon membranes containing 375 cDNA clones. Densitometric analysis confirmed differences in expression (>5-fold) for 97 of the cytokine-related gene products between AAA and adjacent control tissue. Among these, simultaneous upregulation was found in the expression of interleukin (IL)-8 (9-fold) and its receptor, CXCR-2 (11-fold). Thus, the expressions of IL-8 and CXCR-2 were further quantified by real-time RT-PCR. The expression of both the genes was significantly upregulated in both AAA and CAS compared with control regions as followed: IL-8 =0.53±0.16 vs 0.11±0.04 (p<0.01); CXCR-2 =2.04±0.75 vs 0.29±0.10 (p<0.01) in AAA, and IL-8 =1.35 ±0.25 vs 0.60±0.16; CXCR-2 =2.00 ±0.51 vs 0.58±0.21 (p<0.05) in CAS. Under these conditions, the gene expressions of monocyte chemotactic protein-1 and its receptor, CCR-2, were not significantly different in the control and diseased regions of both AAA and CAS. Conclusions Sustained upregulation of IL-8 and CXCR-2 was observed in both AAA and CAS, suggesting the inflammatory process is still active in established dilated and occlusive atherosclerotic diseases. Whether upregulation of this system could be protective or not protective for disease development requires further study. (Circ J 2005; 69: 1490 - 1495)<br>

収録刊行物

  • Circulation Journal

    Circulation Journal 69 (12), 1490-1495, 2005

    一般社団法人 日本循環器学会

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