Enantioseparation of hydroxyeicosatetraenoic acids by hydroxypropyl-gamma-cyclodextrin-modified micellar electrokinetic chromatography

• Kodama, Shuji

• Nakajima, Shota

• Ozaki, Hiromichi

• Takemoto, Ryota

• Itabashi, Yutaka

• Kuksis, Arnis

抄録

Complete resolution of hydroxyeicosatetraenoic acid (HETE) enantiomers was achieved using hydroxypropyl-gamma-cyclodextrin (HP-gamma-CD)-modified MEKC. The optimum running conditions were determined to be utilizing a 30 mM phosphate-15 mM borate buffer (pH 9.0) containing 30 mM HP-gamma-CD and 75 mM SDS as the BGE, application of +30 kV as the effective voltage, and carrying out the experiment at 15 degrees C. The eluents were detected at 235 nm. The method was used successfully for the simultaneous separations of (S)- and (R)-enantiomers of regioisomeric 8-, 11-, 12-, and 15-HETEs. Subsequently, the optimized method was applied to evaluate the stereochemistry of 8- and 12-HETEs from the marine red algae, Gracilaria vermiculophylla and Gracilaria arcuata, respectively. The 8-HETE was found to be a mixture of 98% (R)-enantiomer and 2% (S)-enantiomer, while the 12-HETE was a mixture of 98% (S)-enantiomer and 2% (R)-enantiomer. The present study demonstrates that the HP-gamma-CD-modified MEKC method is simple and sensitive and provides unambiguous information on the configuration of natural and synthetic HETEs.

収録刊行物

• Electrophoresis

  Electrophoresis 37 (23-24), 3196-3205, 2016-12

  Wiley-Blackwell

キーワード

• Enantioseparation
• Gracilaria
• Hydroxyeicosatetraenoic acid
• Hydroxypropyl-gamma-cyclodextrin
• Micellar electrokinetic chromatography

詳細情報

• CRID: 1050564288976241792
• NII論文ID: 120006366494
• NII書誌ID: AA11619422
• ISSN: 15222683
• ISSN: 01730835
• HANDLE: 2115/67771
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles