Anchorless cell surface proteins function as laminin-binding adhesins in Lactobacillus rhamnosus FSMM22

• Aryantini, Ni Putu Desy

• 近藤, 大輔

• Nishiyama, Keita

• Yamamoto, Yuji

• Mukai, Takao

• Sujaya, I Nengah

• 浦島, 匡

• 福田, 健二

抄録

Anchorless cell surface proteins (CSPs) were extracted with 1 M lithium chloride solution from Lactobacillus rhamnosus FSMM22. Loss of the anchorless CSPs resulted in a 2-fold decrease in FSMM22 cells bound to a constitutive extracellular matrix glycoprotein, laminin, in vitro. DNA-binding protein HU, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and 30S ribosomal protein S19 (RpsS) were identified by mass spectrometry in the extract as laminin-binding adhesins. Among the four proteins, RpsS was immunohistochemically confirmed to exist on the cell surface. Our findings strongly suggest that anchorless CSPs can enhance bacterial adhesion to the host.

収録刊行物

• Fems Microbiology Letters

  Fems Microbiology Letters 364 (6), fnx056-, 2017-03

  Oxford University Press

キーワード

• cell surface proteins
• host-microbial interactions
• lactic acid bacteria
• laminin
• probiotics
• ribosomal proteins

詳細情報

• CRID: 1050564287538733312
• NII論文ID: 120006414712
• NII書誌ID: AA00193861
• ISSN: 03781097
• Web Site: https://obihiro.repo.nii.ac.jp/records/4181
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles