Anthocyanin production in suspension cultures of high-producing cells of Euphorbia millii.
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- YAMAMOTO Yoshikazu
- Technical Center, Nippon Paint Co., Ltd.
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- KINOSHITA Yasuhiro
- Technical Center, Nippon Paint Co., Ltd.
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- WATANABE Satomi
- Technical Center, Nippon Paint Co., Ltd.
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- YAMADA Yasuyuki
- Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
抄録
We investigated the optimal conditions for anthocyanin production in suspension cultures of a Euphorbia millii cell strain obtained by successive cell-aggregate selections. A modified Gamborg's solution containing 5 to 7% sucrose (w/v), a 1:16 ratio of NH4+:NO3-, 30 mM of nitrogen source, 10-6 M 2, 4-D, 10-8 M BA, and low concentrations of Fe2+ and SO2-4 greatly increased anthocyanin production. 2, 4-D was superior to NAA in increasing anthocyanin production. In the anthocyanin production medium we established, E. millii cells produced 32 mg anthocyanin per liter per day.
収録刊行物
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- Agricultural and Biological Chemistry
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Agricultural and Biological Chemistry 53 (2), 417-423, 1989
公益社団法人 日本農芸化学会
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キーワード
詳細情報
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- CRID
- 1390282681441462528
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- NII論文ID
- 130000023996
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- COI
- 1:CAS:528:DyaL1MXhslWktL0%3D
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- ISSN
- 18811280
- 00021369
- http://id.crossref.org/issn/00021369
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可