Integrative transformation Aspergillus oryzae with a plasmid containing the Aspergillus nidulans argB gene
-
- GOMI Katsuya
- National Research Institute of Brewing
-
- IIMURA Yuzuru
- National Research Institute of Brewing
-
- HARA Shodo
- National Research Institute of Brewing
書誌事項
- タイトル別名
-
- Integrative transformation of Aspergillus oryzae with a plasmid containing the Aspergillus nidulans argB gene.
- Integrative transformation of Aspergillus oryzae with plasmids containing the Aspergillus nidulans argB gene
- Integrative transformation of Aspergillus oryzae with a plasmid containg the Aspergillus nidulans argB gene
- Integrative transformation of Aspergillus oryzae with a plasmid containing Aspergillus nidulans argB gene
抄録
The transformation of Aspergillus oryzae has been achieved with a plasmid carrying the Aspergillus nidulans argB gene coding for ornithine carbamoyltransferase (OCTase). The frequency of transformation was relatively low (0.7 transformants/μg DNA) but the transformed phenotype was extremely stable for many generations without selective pressure.<br> Southern blot analysis revealed that transformation had occurred by integration of multiple tandem copies of plasmid DNA into the host genome through non-homologous recombination. There was no evidence of the existence of free plasmid in the transformants. The number of integrated copies of the plasmid ranged from 15 to 60. The specific activity of OCTase in the cellfree extract was proportional to the copy number of the plasmid, indicating that most of the integrated argB gene was expressed.
収録刊行物
-
- Agricultural and Biological Chemistry
-
Agricultural and Biological Chemistry 51 (9), 2549-2555, 1987
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282681441204096
-
- NII論文ID
- 130000024895
-
- COI
- 1:CAS:528:DyaL2sXls12qtrc%3D
-
- ISSN
- 18811280
- 00021369
- http://id.crossref.org/issn/00021369
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可