Measurement of tryptophan in peptides by acid hydrolysis in the presence of phenol and its application to the amino acid sequence of a sea anemone toxin.

• MURAMOTO Koji

  School of Fisheries Sciences, Kitasato University

• SUNAHARA Satoshi

  School of Fisheries Sciences, Kitasato University

• KAMIYA Hisao

  School of Fisheries Sciences, Kitasato University

抄録

The addition of phenol (about 1 %) to 6 M HCl largely prevented destruction of tryptophan during hydrolysis of peptides at 110°C for 22hr. Tryptophan recovery depended on the volume of 6 M HCl containing phenol and the concentration of phenol. The maximum tryptophan recovery was 85% for a standard amino acid mixture. The recovery was slightly lower for proteins. This hydrolytic procedure was advantageous for micro amino acid analysis using a conventional highperformance liquid chromatography with a precolumn labeling technique. The method was used in the amino acid sequence analysis of a minor component of sea anemone toxins isolated from Anthopleura fuscoviridis. The toxin consisted of 48 amino acid residues with three tryptophan residues.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 51 (6), 1607-1616, 1987

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390282681441844736
• NII論文ID: 130000025042
• DOI: 10.1271/bbb1961.51.1607
• COI: 1:CAS:528:DyaL2sXkslemu78%3D
• ISSN: 18811280
• ISSN: 00021369
• ISSN: http://id.crossref.org/issn/00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可