Molecular cloning of a cellulase gene from Bacillus subtilis and its expression in Escherichia coli.

• KOIDE Yoshinao

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo Present address: Amano Pharmaceutical Co.

• NAKAMURA Akira

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

• UOZUMI Takeshi

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

• BEPPU Teruhiko

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

抄録

The gene for an endolytic cellulase of Bacillus subtilis was cloned on pBD64 in B. subtilis. The recombinant plasmid, pBC5, contained a 4.8-kb fragment derived from the B. subtilis chromosome, and the transformant overproduced the extracellular enzyme. The inserted fragment was subcloned on pBR322 and the recombinant plasmid, pBRC5, was introduced into Escherichia coli JM105. The E. coli transformant produced the enzyme in both the periplasmic space of cells and the cultural broth.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 50 (1), 233-237, 1986

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390282681442648448
• NII論文ID: 130000026051
• DOI: 10.1271/bbb1961.50.233
• COI: 1:CAS:528:DyaL28XhtVemsL4%3D
• ISSN: 18811280
• ISSN: 00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可