Purification and properties of endopolygalacturonase I from Stereum purpureum, a factor inducing silver-leaf symptoms on apple trees.

• MIYAIRI Kazuo

  Laboratory of Biological Chemistry, Faculty of Agriculture, Hirosaki University

• OKUNO Toshikatsu

  Laboratory of Biological Chemistry, Faculty of Agriculture, Hirosaki University

• SAWAI Ko

  Laboratory of Biological Chemistry, Faculty of Agriculture, Hirosaki University

抄録

Endopolygalacturonase I [EC 3.2.1.15], the major component of endopolygalacturonases causing silver-leaf symptoms, was purified from culture liquids of Stereum purpureum by column chromatographies on CM-52 and Sephadex G-100. The purified enzyme was homogeneous on polyacrylamide gel electrophoresis and ultracentrifugation. The sedimentation coefficient (S_{20, w}) was determined to be 3.21 S, and the molecular weight was estimated to be 40, 000 by gel filtration, 41, 000 by SDS-polyacrylamide gel electrophoresis and 44, 000 by sedimentation equilibrium. The enzyme had an isoelectric point of pH 8.5. The optimal pH of the enzyme was 3.5 for trigalacturonic acid, 4.0 for tetragalacturonic acid, and 4.5 for pectic acid. The enzyme was stable in the range of pH 4.0 to 9.0 and up to 70°C for 30min. The amount of the enzyme which was required to induce silver-leaf symptoms on apple trees was 20μg/tree.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 49 (4), 1111-1118, 1985

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390282681440141952
• NII論文ID: 130000026373
• DOI: 10.1271/bbb1961.49.1111
• COI: 1:CAS:528:DyaL2MXktValt78%3D
• ISSN: 18811280
• ISSN: 00021369
• ISSN: http://id.crossref.org/issn/00021369
• 本文言語コード: en
• データソース種別:
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  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可