Changes in surface charge, respiratory rate and stainability with crystal violet of resting Escherichia coli B cells anaerobically exposed to an alternating current.

  • SHIMADA Keiko
    Department of Industrial Chemistry, Faculty of Engineering, Seikei University
  • SHIMAHARA Kenzo
    Department of Industrial Chemistry, Faculty of Engineering, Seikei University

抄録

Phosphate buffer suspensions of resting Escherichia coli B cells at pH 7.0 were anaerobically exposed to an alternating current (AC) of 50 Hz at a current density of 600 mA/cm2 and 34±3°C. The AC-exposed cells required a higher concentration of a cationic flocculant, poly(methacryloxyethyl-trimethyl-ammonium chloride), to form cell floes than unexposed cells. The electrophoretic mobility of the cells increased after AC-exposure. Oxygen uptake by the exposed cells in a neutral reaction mixture decreased with increasing exposure time. The cells were less easily stained with crystal violet after AC-exposure. These observations show that AC-exposure causes an increase in the amount of the negative surface charge of E. coli cells and changes in some physiological properties.

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詳細情報 詳細情報について

  • CRID
    1390001206464855296
  • NII論文ID
    130000026481
  • DOI
    10.1271/bbb1961.49.405
  • COI
    1:CAS:528:DyaL2MXhs1emu7Y%3D
  • ISSN
    18811280
    00021369
    http://id.crossref.org/issn/00021369
  • 本文言語コード
    en
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

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