Comparison of the multiple agglutinins of the acorn barnacle, Megabalanus rosa.

• MURAMOTO Koji

  School of Fisheries Sciences, Kitasato University

• OGATA Kyoko

  School of Fisheries Sciences, Kitasato University

• KAMIYA Hisao

  School of Fisheries Sciences, Kitasato University

抄録

We investigated the subunit structures of the three agglutinins (BRA-1, BRA-2, and BRA-3) isolated from the coelomic fluid of the acorn barnacle, Megabalanus rosa, by high-speed gel filtration, gel electrophoresis, CD spectra, and peptide mapping. BRA-2 (Mr 140, 000) and BRA-3 (Mr 64, 000) were glycoproteins composed of subunits having two identical basic units cross-linked by disulfide bonds. The basic units of BRA-2 and BRA-3 had molecular weights of 22, 000 and 18, 000, respectively. They did not have a common structure. BRA-1 (Mr 330, 000) was composed of subunits homologous to that of BRA-2. The binding properties of these agglutinins against rabbit erythrocyte ghosts were also investigated after labeling the agglutinins with fluorescein isothiocyanate.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 49 (1), 85-93, 1985

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390282681441288448
• NII論文ID: 130000026666
• DOI: 10.1271/bbb1961.49.85
• COI: 1:CAS:528:DyaL2MXhtFWhsLs%3D
• ISSN: 18811280
• ISSN: 00021369
• ISSN: http://id.crossref.org/issn/00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可