Enzymatic decolorization of melanoid by Coriolus sp. no. 20

• WATANABE Yoshio

  Department of Agricultural Chemistry, Kyushu University

• SUGI Ryozo

  Department of Agricultural Chemistry, Kyushu University

• TANAKA Yonemi

  Department of Agricultural Chemistry, Kyushu University

• HAYASHIDA Shinsaku

  Department of Agricultural Chemistry, Kyushu University

書誌事項

タイトル別名

• Enzymatic decolorization of melanoidin by Coriolus sp. No. 20.
• Enzymatic decolorization of melanoidine by Coriolus sp. no. 20

抄録

Coriolus sp. No. 20 decolorized a melanoidin solution, a decrease of about 80% in darkness under the optimal conditions. This decolorization occurred with an intracellular enzyme which was prepared from an extract of integrated mycelia, and required aeration and some kinds of sugars, particularly glucose and sorbose. The fraction with melanoidin-decolorizing activity was collected and purified by DEAE-cellulose and Sephadex G-200 column chromatographies. The optimal pH and temperature were pH 4.5 and 35°C, respectively. The molecular weight was found to be about 200, 000 by SDS-gel electrophoresis. The purified enzyme was identified as sorbose oxidase; decolorization proceeded in the presence of oxygen and sugars such as maltose, sucrose, lactose, galactose and xylose, besides glucose and sorbose. Glucose in the reaction mixture was converted to gluconic acid. Melanoidin was suggested to be decolorized by the active oxygen formed.

収録刊行物

• Agricultural and Biological Chemistry

  Agricultural and Biological Chemistry 46 (6), 1623-1630, 1982

  公益社団法人 日本農芸化学会

キーワード

• General Agricultural and Biological Sciences
• General Biochemistry, Genetics and Molecular Biology

詳細情報

• CRID: 1390001206465093376
• NII論文ID: 130000027956
• DOI: 10.1271/bbb1961.46.1623
• COI: 1:CAS:528:DyaL38Xks1ynsLo%3D
• ISSN: 18811280
• ISSN: 00021369
• ISSN: http://id.crossref.org/issn/00021369
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可