Cytolytic Toxin Cyt1Aa of<i>Bacillus thuringiensis</i>Synergizes the Mosquitocidal Toxin Mtx1 of<i>Bacillus sphaericus</i>
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- ZHANG Beihua
- Wuhan Institute of Virology, Chinese Academy of Sciences
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- LIU Ming
- Wuhan Institute of Virology, Chinese Academy of Sciences
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- YANG Yankun
- Wuhan Institute of Virology, Chinese Academy of Sciences
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- YUAN Zhiming
- Wuhan Institute of Virology, Chinese Academy of Sciences
書誌事項
- タイトル別名
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- Cytolytic Toxin Cyt1Aa of Bacillus thuringiensis Synergizes the Mosquitocidal Toxin Mtx1 of Bacillus sphaericus
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抄録
Using the shuttle vector pBU4, the mosquitocidal toxin gene mtx1 from Bacillus sphaericus strain SSII-1 was introduced into an acrystalliferous strain of B. thuringiensis both individually and in combination with the accessory protein gene p20 and the cytolytic protein gene cyt1Aa from B. thuringiensis subsp. israelensis. Bioassay results indicated that the recombinants B-pMT4(Mtx1) and B-pMT9(Mtx1), both individually containing mtx1, had moderate toxicities to binary toxin susceptible and binary toxin resistant Culex quinquefasciatus larvae during the vegetative growth stage, but that their toxicities declined rapidly during the sporulation phase. The LC50 values were 2.5 and 4.8 mg/ml respectively, against 3–4 instar susceptible and resistant larvae for the final sporulated cultures of recombinants B-pMT9(Mtx1), and little toxicity was detected for B-pMT4(Mtx1). Meanwhile, the recombinant B-pMPX2(Mtx1+Cyt1Aa) expressing Mtx1, P20 alone, and Cyt1Aa in combination had stable toxicities during both the vegetative phase and the sporulation phase, with a LC50 ranging from 0.45–0.58 mg/ml. Furthermore, expression of Cyt1Aa appeared to enhance the activity of Mtx1 to target mosquito larvae, suggesting a synergism between Cyt1Aa and Mtx1 toxins.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (9), 2199-2204, 2006
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206479633920
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- NII論文ID
- 130000029739
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 8073533
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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