Characterization of the AP Endonucleases from Thermoplasma volcanium and Lactobacillus plantarum: Contributions of Two Important Tryptophan Residues to AP Site Recognition
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- KANEDA Kohichi
- Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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- OHISHI Kaori
- Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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- SEKIGUCHI Junichi
- Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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- SHIDA Toshio
- Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
書誌事項
- タイトル別名
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- Characterization of the AP Endonucleases from <I>Thermoplasma volcanium</I> and <I>Lactobacillus plantarum</I>: Contributions of Two Important Tryptophan Residues to AP Site Recognition
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Escherichia coli AP endonuclease (ExoIII) and its human homolog (APE1) have the sole tryptophan residue for AP site recognition (AP site recognizer) but these residues are at different positions near the catalytic sites. On the other hand, many bacterial AP endonucleases have two tryptophan residues at the same positions of both ExoIII and APE1. To elucidate whether these residues are involved in AP site recognition, the ExoIII homologs of Thermoplasma volcanium and Lactobacillus plantarum were characterized. These proteins showed AP endonuclease and 3′-5′exonculease activities. In each enzyme, the mutations of the tryptophan residues corresponding to Trp-280 of APE1 caused more significant reductions in activities and binding abilities to the oligonucleotide containing an AP site (AP-DNA) than those corresponding to Trp-212 of ExoIII. These results suggest that the tryptophan residue corresponding to Trp-280 of APE1 is the predominant AP site recognizer, and that corresponding to Trp-212 of ExoIII is the auxiliary recognizer.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 70 (9), 2213-2221, 2006
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681456425216
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- NII論文ID
- 130000029741
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD28XhtFSjur7P
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 8073555
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- PubMed
- 16960376
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可