Occurrence of Novel Nonmethylene-Interrupted C<sub>24</sub>Polyenoic Fatty Acids in Female Gonad Lipids of the Limpet<i>Cellana grata</i>

  • KAWASHIMA Hideki
    Bioscience Laboratory, Miyako College, Iwate Prefectural University
  • OHNISHI Masao
    Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine

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  • Occurrence of Novel Nonmethylene-Interrupted C24 Polyenoic Fatty Acids in Female Gonad Lipids of the Limpet Cellana grata

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The occurrence of positional isomers of minor C24 unsaturated fatty acids in female gonad lipids of the limpet Cellana grata was clarified by gas chromatography–mass spectrometry of the combination of their 4,4-dimethyloxazoline and picolinyl ester derivatives. In this study, in addition to 5,9-24:2, 9,15-24:2, 5,9,15-24:3, and 5,9,17-24:3, previously identified, 24:4n-6, 24:5n-3, and four novel nonmethylene-interrupted fatty acids, 9,17-24:2, 9,15,18-24:3, 5,9,15,18-24:4, and 5,9,15,18,21-24:5, were newly recognized. All C24 unsaturated fatty acids detected were present only in triacylglycerols.

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