Glycoform Analysis of Japanese Cedar Pollen Allergen, Cry j 1

• MAEDA Megumi

  Division of Biomolecular Science, Graduate School of Natural Science and Technology, Okayama University

• KAMAMOTO Maiko

  Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

• HINO Katsuhiko

  Fujisaki Institute, Hayashibara Biochemical Laboratory, Inc.

• YAMAMOTO Shigeto

  Fujisaki Institute, Hayashibara Biochemical Laboratory, Inc.

• KIMURA Mariko

  Faculty of Food Culture, Department of Food Systems, Kurashiki Sakuyo University

• OKANO Mitsuhiro

  Department of Otolaryngology-Head and Neck Surgery, Okayama University Graduate School of Medicine and Dentistry

• KIMURA Yoshinobu

  Division of Biomolecular Science, Graduate School of Natural Science and Technology, Okayama University Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University

抄録

In our previous study (Y. Kimura et al., Biosci. Biotechnol. Biochem., 69, 137–144 (2005)), we found that plant complex type N-glycans harboring Lewis a epitope are linked to the mountain cedar pollen allergen Jun a 1. Jun a 1 is a glycoprotein highly homologous with Japanese cedar pollen glycoallergen, Cry j 1. Although it has been found that some plant complex type N-glycans are linked to Cry j 1, the occurrence of Lewis a epitope in the N-glycan moiety has not been proved yet. Hence, we reinvestigated the glycoform of the pollen allergen to find whether the Lewis a epitope(s) occur in the N-glycan moiety of Cry j 1. From the cedar pollen glycoallergen, the N-glycans were liberated by hydrazinolysis and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC. The structures were analyzed by a combination of exo- and endo-glycosidase digestions, sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS). Structural analysis clearly indicated that Lewis a epitope (Galβ1-3(Fucα1-4)GlcNAcβ1-), instead of the Galβ1-4(Fucα1-6)GlcNAc, occurs in the N-glycans of Cry j 1.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 69 (9), 1700-1705, 2005

  公益社団法人 日本農芸化学会

キーワード

• <I>N</I>-glycan
• Lewis a epitope
• pollen allergen
• Japanese cedar pollen
• Cry j 1

詳細情報

• CRID: 1390001206473888000
• NII論文ID: 130000030205
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.69.1700
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7458154
• PubMed: 16195587
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7458154
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.69.1700
• Web Site: https://search.jamas.or.jp/link/ui/2006163396
• 本文言語コード: en
• データソース種別:
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