Gibberellin 2-Oxidases from Seedlings of Adzuki Bean (Vigna angularis) Show High Gibberellin-Binding Activity in the Presence of 2-Oxoglutarate and Co〔2+〕

• PARK Seung-Hyun

  Department of Applied Biological Chemistry, University of Tokyo

• NAKAJIMA Masatoshi

  Department of Applied Biological Chemistry, University of Tokyo

• SAKANE Masayuki

  Department of Applied Biological Chemistry, University of Tokyo

• XU Zheng-Jun

  Rice Institute, Sichuan Agricultural University

• TOMIOKA Katsushi

  Department of Applied Biological Chemistry, University of Tokyo

• YAMAGUCHI Isomaro

  Department of Applied Biological Chemistry, University of Tokyo

書誌事項

タイトル別名

• Gibberellin 2-Oxidases from Seedlings of Adzuki Bean (Vigna angularis) Show High Gibberellin-Binding Activity in the Presence of 2-Oxoglutarate and Co2+
• Gibberellin 2-Oxidases from Seedlings of Adzuki Bean (<i>Vigna angularis</i>) Show High Gibberellin-Binding Activity in the Presence of 2-Oxoglutarate and Co²⁺

抄録

Five full-length cDNA encoding gibberellin 2-oxidases, VaGA2oxA1, VaGA2oxA2, VaGA2oxB1, VaGA2oxB2, and VaGA2oxB3, were cloned from etiolated adzuki bean (Vigna angularis cv. Dainagon) seedlings, and their enzymatic characteristics were examined using recombinant enzymes fused with glutathione S-transferase (GST). Recombinant VaGA2oxA1 (rVaGA2oxA1) and rVaGA2oxA2 showed 2β-hydroxylation activity by converting GA₁, GA₄, GA₉, GA₂₀, GA₄-methyl ester, and 16,17-dihydro-GA₄ to the corresponding 2β-hydroxylated gibberellins, which were identified by GC/MS. rVaGA2oxB1, rVaGA2oxB2, and rVaGA2oxB3 showed similar activity by converting [³H₄]-16,17-dihydro-GA₄ to a metabolite showing an R_f value of 16,17-dihydro-GA₃₄. RNA-blot analysis showed that VaGA2oxA1 and VaGA2oxA2 were the major ones expressed in etiolated hypocotyls. The addition of Co²⁺ instead of Fe²⁺ to the assay medium apparently reduced the enzymatic activity, but increased the binding of [³H₄]-16,17-dihydro-GA₄ to rVaGA2oxA1, indicating the possibility that VaGA2oxs can be detected as gibberellin-binding proteins under certain conditions.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 69 (8), 1498-1507, 2005

  公益社団法人 日本農芸化学会

キーワード

• adzuki bean (<I>Vigna angularis</I>)
• gibberellin
• gibberellin 2-oxidase
• gibberellin-binding protein

詳細情報

• CRID: 1390001206474097152
• NII論文ID: 130000030235
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.69.1498
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7419956
• PubMed: 16116277
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7419956
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.69.1498
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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