Disruption of<i>rpmJ</i>Encoding Ribosomal Protein L36 Decreases the Expression of<i>secY</i>Upstream of the<i>spc</i>Operon and Inhibits Protein Translocation in<i>Escherichia coli</i>

• IKEGAMI Ayao

  Institute of Molecular and Cellular Biosciences, University of Tokyo Department of Life Science, Rikkyo University

• NISHIYAMA Ken-ichi

  Institute of Molecular and Cellular Biosciences, University of Tokyo

• MATSUYAMA Shin-ichi

  Department of Life Science, Rikkyo University

• TOKUDA Hajime

  Institute of Molecular and Cellular Biosciences, University of Tokyo

書誌事項

タイトル別名

• Disruption of rpmJ Encoding Ribosomal Protein L36 Decreases the Expression of secY Upstream of the spc Operon and Inhibits Protein Translocation in Escherichia coli

抄録

The spc operon of Escherichia coli encodes 11 ribosomal proteins and SecY. The secY gene and downstream rpmJ encoding a ribosomal protein, L36, are located distal to the promoter of the spc operon. It has been suggested that the stability of SecY mRNA depends on rpmJ unless a ρ-independent terminator is inserted immediately downstream of secY. Moreover, it has been suggested that RpmJ is dispensable for E. coli. We constructed rpmJ null strains, AY101 (ΔrpmJ::tetA) and AY201 (ΔrpmJ::cat), by replacing rpmJ with tetA, which encodes a membrane protein responsible for tetracycline-resistance, and cat, which encodes a cytoplasmic chloramphenicol acetyltransferase, respectively. Depletion of RpmJ did not inhibit protein synthesis, whereas the growth of AY101 was defective at high temperatures. The level of SecY mRNA decreased significantly in both disruptants even though the ρ-independent terminator was inserted immediately downstream of secY. Some periplasmic proteins were missing in the disruptants with a concomitant increase in the amount of phage shock protein in the inner membrane. These phenotypes caused by the rpmJ null mutation were corrected by a plasmid carrying secY, but not by one carrying rpmJ.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 69 (8), 1595-1602, 2005

  公益社団法人 日本農芸化学会

キーワード

• protein synthesis
• mRNA
• SecY
• ribosomal protein
• RpmJ

詳細情報

• CRID: 1390001206474024064
• NII論文ID: 130000030249
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.69.1595
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7420091
• PubMed: 16116291
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7420091
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.69.1595
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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