Copper-Dependent Production of a<i>Pycnoporus coccineus</i>Extracellular Laccase in<i>Aspergillus oryzae</i>and<i>Saccharomyces cerevisiae</i>
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- HOSHIDA Hisashi
- Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University
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- FUJITA Tsuneyasu
- Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University
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- MURATA Kenji
- Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University
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- KUBO Kanako
- Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University
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- AKADA Rinji
- Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Yamaguchi University
書誌事項
- タイトル別名
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- Copper-Dependent Production of a Pycnoporus coccineus Extracellular Laccase in Aspergillus oryzae and Saccharomyces cerevisiae
この論文をさがす
抄録
Laccase is a multicopper-containing enzyme that catalyzes the oxidation of phenolic compounds. lcc1 cDNA coding for a secretory laccase of Pycnoporus coccineus was expressed under the maltose inducible amyB promoter in Aspergillus oryzae and under the galactose inducible GAL10 promoter in Saccharomyces cerevisiae. Laccase activities, which were undetectable in the absence of copper, were observed by increasing copper concentrations in the media for both systems. The amounts of secreted laccase protein but not lcc1 mRNA increased in proportion to copper concentrations in A. oryzae. The extracellular activities of native A. oryzae amylase and recombinant RNase-T1 expressed from the same amyB promoter in A. oryzae were constant regardless of copper concentrations. Our results indicate that a high copper concentration is required for the production of active laccase in heterologous hosts and that the copper is required for a post-transcriptional process.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 69 (6), 1090-1097, 2005
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681449700224
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- NII論文ID
- 130000030293
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD2MXmtFKmsLo%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 7349478
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- PubMed
- 15973039
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可