<i>Corynebacterium</i>sp. U-96 Contains a Cluster of Genes of Enzymes for the Catabolism of Sarcosine to Pyruvate

  • SUZUKI Haruo
    Division of Biosciences, Graduate School of Fundamental Life Science, Kitasato University Department of Biosciences, School of Science, Kitasato University
  • TAMAMURA Ryou
    Division of Biosciences, Graduate School of Fundamental Life Science, Kitasato University
  • YAJIMA Satoshi
    Department of Biosciences, School of Science, Kitasato University
  • KANNO Miho
    Division of Biosciences, Graduate School of Fundamental Life Science, Kitasato University
  • SUGURO Masaya
    Division of Biosciences, Graduate School of Fundamental Life Science, Kitasato University

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  • Corynebacterium sp. U-96 Contains a Cluster of Genes of Enzymes for the Catabolism of Sarcosine to Pyruvate

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The sarcosine oxidase gene and nearby genes from Corynebacterium sp. U-96 were determined. The genes for serine hydroxymethyltransferase, the β, δ, α, and γ subunits of sarcosine oxidase, serine dehydratase, and 10-formyltetrahydrofolate hydrolase are arranged in this order. This suggests that the bacteria contain a cluster of genes for the catabolism of sarcosine to pyruvate. The possibility that the gene cluster is a merit for the cellular energy demands of the bacteria is discussed. Functional expression of sarcosine oxidase in Escherichia coli was accomplished, but the β subunit and the βδ complex were expressed at a low level as a soluble protein.

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