Insertion of Unmarked DNA Sequences in Multiple Loci of the<i>Bacillus subtilis</i>168 Genome: an Efficient Selection Method

• UOTSU-TOMITA Rie

  Mitsubishi Kagaku Institute of Life Sciences

• KANEKO Shinya

  Mitsubishi Kagaku Institute of Life Sciences

• TSUGE Kenji

  Mitsubishi Kagaku Institute of Life Sciences

• ITAYA Mitsuhiro

  Mitsubishi Kagaku Institute of Life Sciences

書誌事項

タイトル別名

• Insertion of Unmarked DNA Sequences in Multiple Loci of the Bacillus subtilis 168 Genome: an Efficient Selection Method
• Insertion of unmarked sequences in multiple loci of the Bacillus subtilis 168 genome: an efficient selection method

抄録

The construction process of Bacillus subtilis strain with multiple mutations is accelerated by simultaneous use of a positive selection method. All the strains selected by neomycin acquired two simultaneous mutations at different genomic loci. The extremely low rate of false positives was accounted for by employing the improved method.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 69 (5), 1036-1039, 2005

  公益社団法人 日本農芸化学会

キーワード

• <I>Bacillus subtilis</I> genome
• transformation
• inositol
• phleomycin
• pBR322

詳細情報

• CRID: 1390282681452183296
• NII論文ID: 130000030340
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.69.1036
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7322782
• PubMed: 15914929
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7322782
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.69.1036
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可