The N-Terminal Regions of β and γ Subunits Lower the Solubility of Adenosylcobalamin-Dependent Diol Dehydratase
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- TOBIMATSU Takamasa
- Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University
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- KAWATA Masahiro
- Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University
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- TORAYA Tetsuo
- Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University
書誌事項
- タイトル別名
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- The N-Terminal Regions of .BETA. and .GAMMA. Subunits Lower the Solubility of Adenosylcobalamin-Dependent Diol Dehydratase
- N Terminal Regions of ベータ and ガンマ Subunits Lower the Solubility of Adenosylcobalamin Dependent Diol Dehydratase
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Adenosylcobalamin-dependent diol dehydratase is one of essential components of carboxysome-like polyhedral bodies. It exists as a heterohexamer (αβγ)2, and its activity is recovered in a precipitant fraction of Klebsiella oxytoca and overexpressing Escherichia coli cells. Limited proteolysis of the enzyme with trypsin converted the enzyme into a highly soluble form without loss of enzyme activity. The N-terminal amino acid sequencing of the enzyme thus solubilized indicated that the N-terminal 20 and 16 amino acid residues had been removed from the β and γ subunits, respectively. Mutant enzymes with the same N-terminal truncations of either or both of the β and γ subunits were expressed on a high level in E. coli cells. All the mutant enzymes obtained were expressed in a soluble, active form. These results indicate that the N-terminal regions of the β and γ subunits lower the solubility of diol dehydratase. The mutant enzyme with the N-terminal truncations of both β and γ subunits was essentially indistinguishable in catalytic properties from recombinant wild-type enzyme or the enzyme purified from K. oxytoca in a soluble form.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 69 (3), 455-462, 2005
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206473857152
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- NII論文ID
- 130000030381
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 7295499
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- PubMed
- 15784971
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可