Mode of Action of β-Glucuronidase from Aspergillus niger on the Sugar Chains of Arabinogalactan-Protein

• HAQUE Md. Ashraful

  Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University

• KOTAKE Toshihisa

  Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University

• TSUMURAYA Yoichi

  Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University

書誌事項

タイトル別名

• Mode of Action of .BETA.-Glucuronidase from Aspergillus niger on the Sugar Chains of Arabinogalactan-Protein
• Mode of Action of ベータ Glucuronidase from Aspergillus niger on the Sugar Chains of Arabinogalactan Protein
• Mode of Action of β-Glucuronidase from<i>Aspergillus niger</i>on the Sugar Chains of Arabinogalactan-Protein

抄録

A β-glucuronidase purified from a commercial pectolytic enzyme preparation of Aspergillus niger hydrolyzed about half of the 4-O-methyl-glucuronic acid (4-Me-GlcA) residues located at the nonreducing terminals of (1→6)-linked β-galactosyl side chains of the carbohydrate portion of a radish arabinogalactan-protein (AGP) modified by treatment with fungal α-L-arabinosidase. Digestion of the α-L-arabinosidase-treated AGP with exo-β-(1→3)-galactanase released, by exo-fission of β-(1→3)-galactosidic bonds in the backbone chains of the AGP, neutral β-(1→6)-galactooligosaccharides with various chain lengths and their acidic derivatives substituted at their nonreducing terminals with 4-Me-β-GlcA groups. In contrast, successive digestion of the α-L-arabinosidase-treated AGP with β-glucuronidase followed by exo-β-(1→3)-galactanase liberated much higher amounts of β-(1→6)-galactooligomers together with a small portion of short acidic oligomers, mainly 4-Me-β-GlcA-(1→6)-Gal and 4-Me-β-GlcA-(1→6)-β-Gal-(1→6)-Gal. These results indicate that β-glucuronidase acts upon 4-Me-β-GlcA residues in long (1→6)-linked β-galactosyl side chains of the AGP, whereas short acidic side chains survive the attack of the enzyme.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 69 (11), 2170-2177, 2005

  公益社団法人 日本農芸化学会

キーワード

• arabinogalactan-protein (AGP)
• <I>Aspergillus niger</I>
• exo-β-(1→3)-galactanase
• β-glucuronidase
• radish

詳細情報

• CRID: 1390282681449452288
• NII論文ID: 130000030491
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.69.2170
• COI: 1:CAS:528:DC%2BD2MXhtlWnurvI
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7729131
• PubMed: 16306699
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7729131
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.69.2170
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可