Occurrence of Lewis a Epitope in<i>N</i>-Glycans of a Glycoallergen, Jun a 1, from Mountain Cedar (<i>Juniperus ashei</i>) Pollen

  • KIMURA Yoshinobu
    Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University Division of Biomolecular Science, Graduate School of Natural Science and Technology, Okayama University
  • KAMAMOTO Maiko
    Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University
  • MAEDA Megumi
    Division of Biomolecular Science, Graduate School of Natural Science and Technology, Okayama University
  • OKANO Mitsuhiro
    Department of Otolaryngology-Head and Neck Surgery, Okayama University Graduate School of Medicine and Dentistry
  • KINO Kosuke
    Meiji Co.

書誌事項

タイトル別名
  • Occurrence of Lewis a Epitope in N-Glycans of a Glycoallergen, Jun a 1, from Mountain Cedar (Juniperus ashei) Pollen
  • Occurrence of Lewis a epitope in N-glycans of a glycoallergen, Jun a 1, from mountain cedar pollen

この論文をさがす

抄録

We have determined the structures of N-glycans linked to major allergens in the mountain cedar (Juniperus ashei) pollen, Jun a 1. First, two kinds of the pollen glycoallergen (Jun a 1-A and Jun a 1-B) were purified from partially purified Jun a 1 by cation exchange chromatography. The N-glycans were liberated by hydrazinolysis from the two glycoallergens and the resulting sugar chains were N-acetylated and then coupled with 2-aminopyridine. Three pyridylaminated sugar chains were purified by reversed-phase HPLC and size-fractionation HPLC from Jun a 1-A and Jun a 1-B respectively. The structures were determined by a combination of exo- and endo-glycosidase digestions, two dimensional sugar chain mapping, and electrospray ionization mass spectrometry (ESI-MS) analysis. Structural analysis indicated that Lewis a epitope (Galβ1–3(Fucα1–4)GlcNAcβ1–) occurs in the N-glycans of the pollen allergens.

収録刊行物

被引用文献 (15)*注記

もっと見る

参考文献 (21)*注記

もっと見る

詳細情報

問題の指摘

ページトップへ