Pradimicin Resistance of Yeast Is Caused by a Mutation of the Putative<i>N</i>-Glycosylation Sites of Osmosensor Protein Sln1
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- HIRAMOTO Fumitaka
- Biotechnology Research Center, Toyama Prefectural University
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- NOMURA Nobuhiko
- Biotechnology Research Center, Toyama Prefectural University
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- FURUMAI Tamotsu
- Biotechnology Research Center, Toyama Prefectural University
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- IGARASHI Yasuhiro
- Biotechnology Research Center, Toyama Prefectural University
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- OKI Toshikazu
- Biotechnology Research Center, Toyama Prefectural University
書誌事項
- タイトル別名
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- Pradimicin Resistance of Yeast Is Caused by a Mutation of the Putative N-Glycosylation Sites of Osmosensor Protein Sln1
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抄録
Pradimicin, a mannose-binding antifungal antibiotic, induces apoptosis-like cell death in Saccharomyces cerevisiae. Previously we found that the substitution of the 74th amino acid from glycine to cysteine in Ypd1 yields a mutant resistant to pradimicin. In this study, the involvement of a membrane-spanning osomosensor, Sln1, which is located upstream of Ypd1, was investigated. A mutant, sln1 ΔNG, that lacks the putative N-glycosylation sites in the extracellular domain became resistant to pradimicin. On the other hand, the null mutants of Ssk1, Pbs2, and Hog1, which are located downstream of the Sln1 cascade, were sensitive to pradimicin as well as the wild-type strain. In conclusion, pradimicin exerts its fungicidal action with the involvement of Sln1, but the downstream branch, Ssk1 and the HOG pathway, is not involved.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 69 (1), 238-241, 2005
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206476546560
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- NII論文ID
- 130000030582
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 7237632
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- PubMed
- 15665496
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可