Localization of α-Glucosidases 1, 2, and 3 in Organs of European Honeybees, Apis mellifera L., and the Origin of α-Glucosidase in Honey

  • KUBOTA Masaki
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
  • TSUJI Masahisa
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
  • NISHIMOTO Mamoru
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
  • WONGCHAWALIT Jintanart
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
  • OKUYAMA Masayuki
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
  • MORI Haruhide
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
  • MATSUI Hirokazu
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
  • SURARIT Rudee
    Faculty of Dentistry, Mahidol University
  • SVASTI Jisnuson
    Faculty of Science, Mahidol University
  • KIMURA Atsuo
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
  • CHIBA Seiya
    Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

書誌事項

タイトル別名
  • Localization of .ALPHA.-Glucosidases I, II, and III in Organs of European Honeybees, Apis mellifera L., and the Origin of .ALPHA.-Glucosidase in Honey
  • Localization of アルファ Glucosidases 1 2 and 3 in Organs of European Honeybees Apis mellifera L and the Origin of アルファ Glucosidase in Honey
  • Localization of α-Glucosidases I, II, and III in Organs of European Honeybees,<i>Apis mellifera</i>L., and the Origin of α-Glucosidase in Honey

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抄録

Three kinds of α-glucosidases, I, II, and III, were purified from European honeybees, Apis mellifera L. In addition, an α-glucosidase was also purified from honey. Some properties, including the substrate specificity of honey α-glucosidase, were almost the same as those of α-glucosidase III. Specific antisera against the α-glucosidases were prepared to examine the localization of α-glucosidases in the organs of honeybees. It was immunologically confirmed for the first time that α-glucosidase I was present in ventriculus, and α-glucosidase II, in ventriculus and haemolymph. α-Glucosidase III, which became apparent to be honey α-glucosidase, was present in the hypopharyngeal gland, from which the enzyme may be secreted into nectar gathered by honeybees. Honey may be finally made up through the process whereby sucrose in nectar, in which glucose and fructose also are naturally contained, is hydrolyzed by secreted α-glucosidase III.

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