Transglycosylation Activity of Dictyoglomus thermophilum Amylase A
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- NAKAJIMA Masahiro
- Department of Biotechnology, University of Tokyo
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- IMAMURA Hiromi
- Department of Biotechnology, University of Tokyo
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- SHOUN Hirofumi
- Department of Biotechnology, University of Tokyo
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- HORINOUCHI Sueharu
- Department of Biotechnology, University of Tokyo
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- WAKAGI Takayoshi
- Department of Biotechnology, University of Tokyo
Bibliographic Information
- Other Title
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- Transglycosylation Activity of<i>Dictyoglomus thermophilum</i>Amylase A
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Abstract
Amylase A from Dictyoglomus thermophilum is a thermophilic enzyme and has about 40% identity with 4-α-glucanotransferase (GTase) from Thermococcus litoralis, and both of these enzymes belong to family 57 glycosyl hydrolase. Since the transglycosylation activity of T. litoralis GTase has been well characterized, the substrate specificity and reaction products of amylase A from D. thermophilum were examined. α-1,4 Glucan was produced from maltooligosaccharides, and glucoamylase-resistant molecules (cycloamyloses) were produced from longer chain amylose (average molecular mass 200 kDa). It has been reported that amylase A from D. thermophilum hydrolyzes starch, but in this study it was found that the enzyme was also able to use maltooligosaccharides and long chain amylose as substrate and has transglycosylation activity.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 68 (11), 2369-2373, 2004
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206474534528
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- NII Article ID
- 130000030928
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 7173348
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- PubMed
- 15564678
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed