Cloning of the Elk Common Glycoprotein α-subunit and the FSH and LH β-subunit cDNAs

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  • CLARK Rena J.
    Department of Veterinary Biomedical Sciences, Gynecology and Reproductive Sciences, University of Saskatchewan, Royal University Hospital
  • FURLAN Michael A.
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of Saskatchewan, Royal University Hospital
  • CHEDRESE P. Jorge
    Department of Obstetrics, Gynecology and Reproductive Sciences, University of Saskatchewan, Royal University Hospital

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タイトル別名
  • Cloning of the Elk Common Glycoprotein .ALPHA.-subunit and the FSH and LH .BETA.-subunit cDNAs
  • Cloning of the Elk Common Glycoprotein アルファ subunit and the FSH and LH ベータ subunit cDNA s

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We report the nucleotide and deduced amino acid sequences of the pituitary glycoprotein hormone common α-subunit, and the FSH and LH β-subunits of the elk (Cervus elaphus). These sequences were obtained by RT-PCR of pituitary gland polyadenylated RNA. A partial genomic fragment of the elk follicle stimulating hormone β-subunit was also amplified from genomic DNA and sequenced. These sequences show high similarity to other related domestic species, including sika deer, ovine, bovine, porcine and equine. The presently reported cDNAs were used as probes for Northern analysis of pituitary gland and muscle samples from elk and sheep. Mature mRNA transcripts of approximately 700, 1700 and 550 bases were expressed in the pituitary gland but not in muscle tissue, for the glycoprotein hormone common α-subunit and FSH and LH β-subunits, respectively. These observations are consistent with reports for other related species. The genomic fragment of elk FSH β-subunit revealed an intronic microsatellite of 6 CT repeats, which differs from the 19 CT repeats documented for the sheep genomic sequence. We propose that differences in the CT repeats of the elk FSH β-subunit gene may be used as a marker for elk parentage testing. The elk DNA sequences presently reported represent a unique addition to the growing collection of the pituitary hormone genes for various vertebrates, which will facilitate evolutionary and phylogenetic studies of the gonadotropin genes. In addition, this information and the cloned cDNAs will be useful for studying seasonal expression of the elk pituitary glycoprotein genes.<br>

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