Immunolocalization of Steroidogenic Enzymes P450scc, 3.BETA.HSD, P450c17, and P450arom in Goettingen Miniature Pig Testes

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  • WENG Qiang
    Faculty of Biological Science and Technology, Beijing Forestry University Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology
  • MEDAN Mohamed S.
    Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology Department of Theriogenology, Faculty of Veterinary Medicine, Suez Canal University
  • WATANABE Gen
    Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology United Graduate School of Veterinary Sciences, Gifu University
  • TSUBOTA Toshio
    United Graduate School of Veterinary Sciences, Gifu University Laboratory of Zoo and Wildlife Medicine, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University
  • TANIOKA Yoshikuni
    Central Institute for Experimental Animals
  • TAYA Kazuyoshi
    Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology United Graduate School of Veterinary Sciences, Gifu University

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Other Title
  • 雄ゲッチンゲンミニブタ精巣におけるステロイドホルモン合成酵素P450scc,P450c17,3βHSDおよびP450aromの免疫局在
  • Immunolocalization of Steroidogenic Enzymes P450scc, 3βHSD, P450c17, and P450arom in Gottingen Miniature Pig Testes
  • Immunolocalization of Steroidogenic Enzymes P450scc 3 ベータ HSD P450c17 and P450arom in Gottingen Miniature Pig Testes

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Abstract

The objective of this study was to investigate immunolocalization of steroidogenic enzymes in Göttingen miniature (GM) pig testes. Testes of 6 adult GM pigs were obtained in September 1996 (n=2), February (n=2) and June (n=2), 1997. Steroidogenic enzymes were immunolocalized using polyclonal antisera raised against bovine adrenal cholesterol side-chain cleavage cytochrome P450 (P450scc), human placental 3β-hydroxysteroid dehydrogenase (3βHSD), porcine testicular 17α-hydroxylase cytochrome P450 (P450c17), and human placental aromatase cytochrome P450 (P450arom). Histologically, all types of spermatogenic cells including mature-phase spermatozoa in seminiferous tubules were observed in all testes throughout the year. Moreover, P450scc, 3βHSD, P450c17and P450arom were identified in Leydig cells but not in Sertoli cells of all testes. These results suggested that adult GM pig testes have the ability to produce germ cells throughout the year, and the synthesis of progestin, androgen and estrogen occurs in the Leydig cells of GM pig testes.<br>

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