Transcriptional Activity of the 5' Upstream Region of the Porcine Glycoprotein Hormone .ALPHA. Subunit Gene
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- AIKAWA Satoko
- Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, School of Agriculture, Meiji University
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- SUSA Takao
- Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, School of Agriculture, Meiji University
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- SATO Takanobu
- Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, School of Agriculture, Meiji University
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- KITAHARA Kousuke
- Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, School of Agriculture, Meiji University
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- KATO Takako
- Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, School of Agriculture, Meiji University
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- KATO Yukio
- Laboratory of Molecular Biology and Gene Regulation, Department of Life Science, School of Agriculture, Meiji University
抄録
Gene expression of the porcine glycoprotein hormone α subunit (p-αGSU) was examined in LβT2 cells, which were established from the anterior pituitary lobe of the immortalized transgenic mouse and produce αGSU, and in CHO cells cloned from Chinese hamster ovaries. Expression of the reporter gene fused with p-αGSU gene upstream in LβT2 cells showed that the distal regions -540/-240 and -798/-541 are important for the activation of gene expression. In contrast, the transcriptional activity of the distal region of p-αGSU gene was repressed in CHO cells. The region -540/-240 contains an adequate enhancer, called pituitary glycoprotein hormone basal element, whereas the region -798/-541 has no distinguished element. Transfection of the expression vector containing cDNA of a pan-pituitary activator, Ptx1, whose putative binding sites are present scatted in the distal region of the p-αGSU gene, revealed unexpectedly that this factor significantly suppressed the expression of p-αGSU gene in LβT2 cells, indicating that Ptx1 is unrelated to the upregulation in the region -798/-541. Thus, this study demonstrated for the first time that the distal region -798/-541of the p-αGSU gene is indispensable for prominent expression of this gene in which an as yet unidentified factor may participate.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 51 (1), 117-121, 2005
公益社団法人 日本繁殖生物学会