Preparation of Ethyl Magnesium Bromide for Regiospecific Analysis of Triacylglycerols

• Ando Yasuhiro

  Laboratory of Marine Bioresources Chemistry, Division of Marine Life Science, Faculty of Fisheries Sciences, Hokkaido University

• Tomita Yuki

  School of Fisheries Sciences, Hokkaido University

• Haba Yusuke

  Graduate School of Fisheries Sciences, Hokkaido University

抄録

This paper presents a procedure for preparation of a Grignard reagent, ethyl magnesium bromide, used for partial deacylation of triacylglycerols (TAG) in their regiospecific analysis. Magnesium turnings were reacted with ethereal solution of bromoethane in a screw-capped test tube to synthesize 2 mL of 1 M ethyl magnesium bromide. Continuously stirred with a vortex mixer, the reaction smoothly proceeded at room temperature. Regiospecific analysis of 1,3-distearoyl-2-oleoylglycerol using this product showed that fatty acid compositions of the sn-1(3) and sn-2 positions were contaminated by less than 2 mol% of fatty acids migrated from isomeric positions. The analyses of lard and cod liver/mackerel oil TAG showed typical distribution patterns of 16:0, 22:5n-3 and 22:6n-3 in pig and fish depot TAG. These results confirmed the view that the freshly prepared reagent is usable for regiospecific analysis of TAG.<br>

収録刊行物

• Journal of Oleo Science

  Journal of Oleo Science 57 (8), 459-462, 2008

  公益社団法人 日本油化学会

キーワード

• ethyl magnesium bromide
• regiospecific analysis
• triacylglycerol

詳細情報

• CRID: 1390001204092148096
• NII論文ID: 130000055383
• NII書誌ID: AA11503337
• DOI: 10.5650/jos.57.459
• ISSN: 13473352
• ISSN: 13458957
• NDL書誌ID: 9579417
• Web Site: http://id.ndl.go.jp/bib/9579417
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9579417
• Web Site: https://www.jstage.jst.go.jp/article/jos/57/8/57_8_459/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可