Isolation and characterization of two homeodomain leucine zipper genes from the dioecious plant Silene latifolia.

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  • Ageez Amr
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo
  • Matsunaga Sachihiro
    Department of Biotechnology, Graduate School of Engineering, Osaka University
  • Uchida Wakana
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo
  • Sugiyama Ryuji
    Department of Biological Sciences, Graduate School of Science, University of Tokyo
  • Kazama Yusuke
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo
  • Kawano Shigeyuki
    Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo

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Abstract

Homeodomain leucine zipper (HD-Zip) genes encode transcription factors that are characterized by both a homeodomain and a leucine zipper motif. Two HD-Zip genes were isolated from cDNA of the male flower bud of the dioecious plant Silene latifolia. The two isolated genes, SlHDL1 and SlHDL2, encode proteins with the characteristics of HD-Zip transcription factors belonging to HD-Zip classes I and II, respectively. The expression patterns of SlHDL1 and SlHDL2 throughout the floral developmental stages were studied using real-time PCR and in situ hybridization. SlHDL1 is specifically expressed in the outermost layer of the anthers and gynoeciums with a patchy pattern in the inner layers, suggesting that the product of SlHDL1 plays a role in the early developmental stage of the epidermal tissues of these floral organs. Its expression pattern in the anthers and gynoeciums suggests an involvement in differentiation of the reproductive organs. On the other hand, real-time PCR revealed accumulation of SlHDL2 transcripts in the anther and pollen grains of the male flower. These results suggest that SlHDL1 and SlHDL2 regulate specific targets in restricted regions leading to floral organ differentiation in S. latifolia.<br>

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