Characterization of the S-RNase promoters from sweet cherry (Prunus avium L.)

• Ishizaka Takako

  JST, Regional Joint Research Project of Yamagata Prefecture

• Nakano Hideaki

  Yamagata Prefecture Agricultural Experiment Station

• Suzuki Takashi

  Faculty of Education, Yamagata University

• Kitashiba Hiroyasu

  JST, Regional Joint Research Project of Yamagata Prefecture

抄録

Genomic DNA fragments containing the S₃-, S₄-, and S₆-RNase genes were isolated from the sweet cherry (Prunus avium L.) and sequenced. Comparison of the 5'-flanking sequences of these three S-RNases indicated that a highly conserved region (designated CR) existed just upstream from the putative TATA boxes. We postulate that CR contains cis-regulatory element(s) involved in pistil expression. To examine the activity of the isolated S-RNase promoters of sweet cherry in the pistil, we transiently introduced approximately 650-bp fragments of the S₄- and S₆-RNase promoters fused to β-glucuronidase (GUS) gene into the pistil of the petunia using a particle bombardment technique. Histochemical analysis showed that the 5'-flanking region of each S-RNase was active in the pistil. This suggests that cis-regulatory element(s) for pistil-specific expression may exist(s) within the 650-bp region upstream from the TATA box in the sweet cherry S-RNase promoter.<br>

収録刊行物

• Genes & Genetic Systems

  Genes & Genetic Systems 78 (2), 191-194, 2003

  日本遺伝学会

キーワード

• 5'-flanking region
• Prunus avium L.
• self-incompatibility
• S-RNase
• sweet cherry

詳細情報

• CRID: 1390282680449695232
• NII論文ID: 130000060774
• NII書誌ID: AA11077421
• DOI: 10.1266/ggs.78.191
• COI: 1:CAS:528:DC%2BD3sXmtVClurg%3D
• ISSN: 18805779
• ISSN: 13417568
• NDL書誌ID: 6536950
• PubMed: 12773819
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6536950
• Web Site: http://www.jstage.jst.go.jp/article/ggs/78/2/78_2_191/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可